Proteomic analysis

Abstract | Chicken model of steroid-induced bone marrow ...

by Wing Chan

We compared the histologic features of the femoral marrow of eight methylprednisolone (MP)-treated chickens with those of three control chickens and assessed differential proteins with 2-dimensional gel electrophoresis and differential proteins... These were serum amyloid P-component precursor, zinc finger protein 28, endothelial zinc finger protein 71, T-box transcription factor 3, cyclin-dependent kinase inhibitor 1, myosin 1D, dimethylaniline monooxygenase, and two uncharacterized proteins. In our study, 13 proteins in the control and MP-induced groups were differently expressed and nine protein spots showed marked threefold downregulation after 19 weeks of MP treatment. Conclusions Proteomic profiling can be a useful dynamic approach for detecting protein expression in MP-induced adipogenesis of the femur in chickens. In this observational study, we aimed to determine the protein expression relating to steroid-induced adipogenesis of femoral bone marrow with use of a chicken model. Abstract (provisional) . Background Steroid-induced adipogenesis increases fat-cell volume and pressure in bone marrow.

Source: Abstract | Chicken model of steroid-induced bone marrow ...

Proteome Analysis Part 01

A presentation in proteome analysis in molecular biology presented by a team of veterinary medicine first year students of Cairo University Egypt ...

Proteomic analysis of purified Newcastle disease virus particles

21.05.12

Newcastle disease virus (NDV) is an enveloped RNA virus, bearing severe economic losses to the poultry industry worldwide. Previous virion proteomic studies have shown that enveloped viruses carry multiple host cellular proteins both internally and externally during their life cycle.

To address whether it also occurred during NDV infection, we performed a comprehensive proteomic analysis of highly purified NDV La Sota strain particles.

Results: In addition to five viral structural proteins, we detected thirty cellular proteins associated with purified NDV La Sota particles. The identified cellular proteins comprised several functional categories, including cytoskeleton proteins, annexins, molecular chaperones, chromatin modifying proteins, enzymes-binding proteins, calcium-binding proteins and signal transduction-associated proteins.

Among these, three host proteins have not been previously reported in virions of other virus families, including two signal transduction-associated

Source: 7thSpace Interactive (press release)

how many micrograme/microliter of bacterial protein needed to run a good gel electrophoresis?

Aug 22, 2008 by TOBI T | Posted in Infectious Diseases

the protein bands will be cut and used for proteomic analysis

Protein concentration of 0.2

Anne2 | Aug 22, 2008

Another biology question!?

Mar 10, 2008 by Tina J | Posted in Biology

How and why is proteomic pattern analysis important in the diagnosis of disease?

disease conditions can result in the expression of particular characteristic proteins. The is especially true with some cancers. The field of proteomics (study of protein expression) is emerging as an important diagnostic tool.

To quote

open_skyz | Mar 10, 2008