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Using the Geneaid gSYNC DNA Mini Kit, high quality Genomic DNA from whole blood can be purified in less than 20 minutes. www.geneaid.com http www ...
SAN DIEGO, Apr 18, 2012 (BUSINESS WIRE) -- Molecular Response (MRL), a privately held molecular diagnostic services company and a leader in identification of target populations for high value therapeutics, today announced the availability of genomic DNA from the world's largest bank of viable cryopreserved tumor cells.
Genomic DNA is a key component to understanding cancer, a disease characterized by DNA sequence abnormalities. Genomic DNA from these tumors can be used for sequencing, mutation and SNP detection, population mutation frequency analysis, and copy number determination. The large bank of specimens available at Molecular Response will greatly reduce the amount of time spent by researchers in locating significant sets of specimens to use in studies.
The genomic DNA available at Molecular Response is isolated from over 12,000 individual tumors, representing over 60 different cancer indications, collected from a de-identified US population over the last 20 years. With cryopreserved tumor cells as the source of DNA, Molecular Response offers quality genomic DNA that has not been subjected to the adverse effects of formalin fixation. Additionally, since the tumors have been disaggregated, the genomic DNA is representative of the entire sample, correcting for intratumor heterogeneity. Each specimen is annotated by cancer indication, gender, age, collection site, clinical diagnosis, histology, and pathology review.
It binds with non-aqueous compounds to separate from aquaeous compounds. This would include certain proteins, and most lipids. Since polar and non-polar substances don't mix, the non-aqueous substances that dissolve in the chloroform separate in a different
I have a protocol for nuclear DNA isolation, and would like to ensure that there is no contamination from organelle DNA. Is there a quick and easy way of quantifying nuclear DNA versus organelle? DNA?
if you have a qPCR machine, then do qPCR on both genomic and mitochondrial sequences. There will always be a little contamination but you can at least quantify it then.
(regular PCR of course will detect even a single molecule of contaminant,
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